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Hi Durga prasad, If you would like to use an external wifi adapter for the hotspot instead of the built in wifi then you just need to change the device from wlan0 to wlan1

in /etc/hostapd.conf
interface=wlan0 to interface=wlan1

in /etc/dnsmasq.conf
#AutoHotspot config
interface=wlan0
to
#AutoHotspot config
interface=wlan1

in /usr/bin/autohotspotN

wifidev="wlan0" #device name to use. Default is wlan0.
ethdev="eth0" #Ethernet port to use with IP tables
#use the command: iw dev ,to see wifi interface name
to

wifidev="wlan1" #device name to use. Default is wlan0.
ethdev="eth0" #Ethernet port to use with IP tables
#use the command: iw dev ,to see wifi interface name

Those changes should use the ALFA Wifi Adapter instead of the internal wifi.